Journal: Bioscience Reports

Article Title: CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

doi: 10.1042/BSR20203459

Figure Lengend Snippet: ( A ) Top 10 terms in GO analysis ( P <0.05). ( B ) Enriched terms in KEGG pathway analysis ( P <0.05). ( C ) Top three PPI networks of CXCL10, KRT, and ZAP70 modules. The network includes three modules and 14 nodes showing the association between tumor microenvironment-related genes and PTC. ( D ) Bar chart signifying important genes (degree>10) in the PPI network.

Article Snippet: After overnight incubation with the rabbit monoclonal anti-CXCL10 antibody (1:100 dilution, BOSTER, Wu Han, China, BA4723) at 4°C, 120-point PTC tissue microarrays were washed with PBS and incubated with broad-spectrum secondary antibodies.

Techniques:

Journal: Bioscience Reports

Article Title: CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

doi: 10.1042/BSR20203459

Figure Lengend Snippet: ( A ) Correlations between CXCL10 and differentially expressed genes in PTC. ( B and C ) Heatmaps indicate the top 50 genes positively and negatively correlated with CXCL10 in PTC. Red refers to positively correlated genes and green refers to negatively correlated genes. Statistical analysis was performed using Pearson’s test. GO function ( D ) and KEGG pathway ( E ) analyses of CXCL10.

Article Snippet: After overnight incubation with the rabbit monoclonal anti-CXCL10 antibody (1:100 dilution, BOSTER, Wu Han, China, BA4723) at 4°C, 120-point PTC tissue microarrays were washed with PBS and incubated with broad-spectrum secondary antibodies.

Techniques:

Journal: Bioscience Reports

Article Title: CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

doi: 10.1042/BSR20203459

Figure Lengend Snippet: Diseases related to CXCL10 (Open Targets)

Article Snippet: After overnight incubation with the rabbit monoclonal anti-CXCL10 antibody (1:100 dilution, BOSTER, Wu Han, China, BA4723) at 4°C, 120-point PTC tissue microarrays were washed with PBS and incubated with broad-spectrum secondary antibodies.

Techniques:

Journal: Bioscience Reports

Article Title: CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

doi: 10.1042/BSR20203459

Figure Lengend Snippet: Gene set enrichment analysis (GSEA) of CXCL10 gene expression

Article Snippet: After overnight incubation with the rabbit monoclonal anti-CXCL10 antibody (1:100 dilution, BOSTER, Wu Han, China, BA4723) at 4°C, 120-point PTC tissue microarrays were washed with PBS and incubated with broad-spectrum secondary antibodies.

Techniques: Gene Expression

Journal: Bioscience Reports

Article Title: CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

doi: 10.1042/BSR20203459

Figure Lengend Snippet: The kinase target networks of CXCL10 in PTC (LinkedOmics)

Article Snippet: After overnight incubation with the rabbit monoclonal anti-CXCL10 antibody (1:100 dilution, BOSTER, Wu Han, China, BA4723) at 4°C, 120-point PTC tissue microarrays were washed with PBS and incubated with broad-spectrum secondary antibodies.

Techniques:

Journal: Bioscience Reports

Article Title: CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

doi: 10.1042/BSR20203459

Figure Lengend Snippet: PPI networks of CXCL10 and related genes (GeneMANIA)

Article Snippet: After overnight incubation with the rabbit monoclonal anti-CXCL10 antibody (1:100 dilution, BOSTER, Wu Han, China, BA4723) at 4°C, 120-point PTC tissue microarrays were washed with PBS and incubated with broad-spectrum secondary antibodies.

Techniques:

Journal: Bioscience Reports

Article Title: CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

doi: 10.1042/BSR20203459

Figure Lengend Snippet: (CXCL10 protein levels were higher in tumor-adjacent tissues compared to PTC tissues; 400× magnification).

Article Snippet: After overnight incubation with the rabbit monoclonal anti-CXCL10 antibody (1:100 dilution, BOSTER, Wu Han, China, BA4723) at 4°C, 120-point PTC tissue microarrays were washed with PBS and incubated with broad-spectrum secondary antibodies.

Techniques:

Journal: Bioscience Reports

Article Title: CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

doi: 10.1042/BSR20203459

Figure Lengend Snippet: Relationship between CXCL10 and various infiltrating immune cells (TIMER)

Article Snippet: After overnight incubation with the rabbit monoclonal anti-CXCL10 antibody (1:100 dilution, BOSTER, Wu Han, China, BA4723) at 4°C, 120-point PTC tissue microarrays were washed with PBS and incubated with broad-spectrum secondary antibodies.

Techniques:

Journal: Bioscience Reports

Article Title: CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

doi: 10.1042/BSR20203459

Figure Lengend Snippet: Correlation analysis between CXCL10 and gene biomarkers of immune cells in THCA (TIMER)

Article Snippet: After overnight incubation with the rabbit monoclonal anti-CXCL10 antibody (1:100 dilution, BOSTER, Wu Han, China, BA4723) at 4°C, 120-point PTC tissue microarrays were washed with PBS and incubated with broad-spectrum secondary antibodies.

Techniques:

Journal: Bioscience Reports

Article Title: CXCL10 is a potential biomarker and associated with immune infiltration in human papillary thyroid cancer

doi: 10.1042/BSR20203459

Figure Lengend Snippet: Drug resistance analysis of the central gene, CXCL10, using GSCALite

Article Snippet: After overnight incubation with the rabbit monoclonal anti-CXCL10 antibody (1:100 dilution, BOSTER, Wu Han, China, BA4723) at 4°C, 120-point PTC tissue microarrays were washed with PBS and incubated with broad-spectrum secondary antibodies.

Techniques:

Journal: The Journal of general virology

Article Title: Equine herpesvirus 1 infection orchestrates the expression of chemokines in equine respiratory epithelial cells.

doi: 10.1099/jgv.0.001317

Figure Lengend Snippet: Fig. 1. Expression patterns of CXCL9 and CXCL10 in mock-, abortigenic (N752) EHV1, or neurovirulent (D752) EHV1-inoculated ERECs. The left panel shows representative confocal images of the immunofluorescence staining, with (a) CXCL9 or (b) CXCL10 stained in green (FITC), viral proteins stained in red (TR), and nuclei counterstained in blue (Hoechst) in mock- or EHV1-inoculated ERECs, at 24 h p.i. Bars, 75 µm. The right panel shows the intensity of CXCL9 or CXCL10 signal in the ERECs at the level randomly chosen for viral plaques. Data are represented as means+sd, and ns indicates no statistically significant differences. *Statistically significant differences at P<0.05. Experiments were performed with ERECs isolated from three individual horses.

Article Snippet: CXCL9 and CXCL10 were visualized by incubating with a rabbit polyclonal anti-CXCL9 antibody (IgG; GeneTex) (1:50 in DPBS) or a rabbit polyclonal anti-CXCL10 antibody (IgG; Kingfisher Biotech) (1:50 in DPBS) followed by an FITCconjugated goat anti-rabbit IgG antibody (1:100 in DPBS) (Molecular Probes).

Techniques: Expressing, Immunofluorescence, Staining, Isolation